Insertion junction: CLIP2.001763_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.001763
Chromosome:chromosome 3
Location:4711258
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g177450 (1 of 1) IPR003729//IPR022772//IPR024053 - Bifunctional nuclease domain // von Hippel-Lindau disease tumour suppressor, beta/alpha domain // von Hippel-Lindau disease tumour suppressor, beta domain 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGTGCAAATCGAGCAGGTGATACCAGAGGTACACACAGTGCGCCAACATA
Internal bar code:GCTTTATAAAGGGATCGGTCTA

Confirmation - LEAP-Seq

LEAP-Seq distance:5075
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:49
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:49

Suggested primers for confirmation by PCR

Suggested primer 1:ATAGCGCATACCGTGACTGG
Suggested primer 2:TGGGGTCTGGCACTTTGTTT