| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.001832 |
| Chromosome: | chromosome 10 |
| Location: | 2940221 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g440000 | RAA8,OPR120 | psaA mRNA trans-splicing factor | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGGCTGCTAGCCGTGGCAGCAGCCGGCACAGCTCGCCACACGAGGGCGG |
| Internal bar code: | AGCGGCCTTTATCGTATATACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5834 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 6 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCTTGCGTGTCTGGACAT |
| Suggested primer 2: | TGCTGCTACTGGTGCTACTG |