| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.002012 |
| Chromosome: | chromosome 5 |
| Location: | 3192937 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g232200 | NDA3,LCI18 | Mitochondrial NADH dehydrogenase; (1 of 1) PTHR22912:SF180 - 3-PHENYLPROPIONATE/CINNAMIC ACID DIOXYGENASE FERREDOXIN--NAD(+) REDUCTASE COMPONENT | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTATTCCCTGTCGAGGTGGAAGGGTTTCTTCTCGAAATGCTGCCACACAG |
| Internal bar code: | CGGGGTGATCCAAATTTTATGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2345 |
| LEAP-Seq percent confirming: | 25.8621 |
| LEAP-Seq n confirming: | 15 |
| LEAP-Seq n nonconfirming: | 43 |
| LEAP-Seq n unique pos: | 58 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCCAAGAGGTTGTCCAGCG |
| Suggested primer 2: | TGGCATTATCGGGTACTCGC |