| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.002068 |
| Chromosome: | chromosome 7 |
| Location: | 292007 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g314150 | ZDS,ZDS1 | (1 of 1) 1.3.5.6 - 9,9'-di-cis-zeta-carotene desaturase / Zeta-carotene desaturase; Zeta-carotene desaturase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCAGTGCTCGTATACTGTATAATACAAGTAGAGTCAGTGCTTGCTTAATT |
| Internal bar code: | ACGGGTCAAGTCAGAAGTAATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1448 |
| LEAP-Seq percent confirming: | 91.3043 |
| LEAP-Seq n confirming: | 21 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGGAGGCTAAACCCACAAT |
| Suggested primer 2: | CCACCGCAGTACCGTTACTT |