| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.002176 |
| Chromosome: | chromosome 1 |
| Location: | 2360637 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g013300 | DEG10 | Deg protease; (1 of 1) PTHR22939:SF87 - DO-LIKE 15 PROTEIN-RELATED | 3'UTR |
| Cre01.g013350 | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTAGATGCACAGCGCTCCTGAGCACCGGCACACGCGATCGTGACGCCGC |
| Internal bar code: | TTAAACTGAGACACTAAAAGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1236 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 31 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTGTGCTCCGCTAGTTTGG |
| Suggested primer 2: | GTGTCAGGACTCAGGAAGGC |