Insertion cassette: | CIB2 |
Side of cassette: | 3' truncated? |
Strand: | - |
Strain: | CLIP2.002198 |
Chromosome: | chromosome 3 |
Location: | 4426200 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre03.g175250 | SQS,SQS1 | Squalene synthase; (1 of 1) 2.5.1.21 - Squalene synthase / Squalene synthetase | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAACAGCCGCACGCTCTACCTACTGAGCTATAGAAGCCCCGCACCAGCCA |
Internal bar code: | GAGCGAGACCAGTTCAAGGGCG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1207 |
LEAP-Seq percent confirming: | 95.2381 |
LEAP-Seq n confirming: | 40 |
LEAP-Seq n nonconfirming: | 2 |
LEAP-Seq n unique pos: | 42 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GTCAAGGCAGGTGGTATGCT |
Suggested primer 2: | ATGCAGCTGGTGGGTAAGAC |