Insertion junction: CLIP2.002204_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.002204
Chromosome:chromosome 10
Location:1182789
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g425900 LHCA5 (1 of 2) K08910 - light-harvesting complex I chlorophyll a/b binding protein 4 (LHCA4); Light-harvesting protein 5 of photosystem I CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTCTACGAGGCTGGCCTGCCCCAGAACCTGCCCGAGCCCTTCACCAACA
Internal bar code:GGGATTACCAGCGACGATCGTT

Confirmation - LEAP-Seq

LEAP-Seq distance:1934
LEAP-Seq percent confirming:94.2857
LEAP-Seq n confirming:33
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:35

Suggested primers for confirmation by PCR

Suggested primer 1:GCAAACTGTCAGCACCTGTG
Suggested primer 2:CCCGGTCATACAAACCACCA