Insertion junction: CLIP2.002284_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.002284
Chromosome:chromosome 3
Location:2154870
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g156900 LHCBM5 (1 of 6) K08912 - light-harvesting complex II chlorophyll a/b binding protein 1 (LHCB1); Light-harvesting Chlorophyll a/b binding protein of LHCII 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAGGTACCTAAGTGCGCTTGCTTGCCCCTAACTTGCAGCGGGCACGTGTA
Internal bar code:CAAGTATTTGAATCGTTTGCAT

Confirmation - LEAP-Seq

LEAP-Seq distance:2759
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:38
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:38

Suggested primers for confirmation by PCR

Suggested primer 1:TAAGGGTCGAACGAGGGGAT
Suggested primer 2:TGTACACGCTGCTGTGCATA