| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.002379 |
| Chromosome: | chromosome 17 |
| Location: | 3734063 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g726350 | (1 of 1) IPR000104//IPR002794 - Antifreeze protein, type I // Protein of unknown function DUF92, TMEM19 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATTTCCCCATCAACTTGGCAGCAACGAACATCGCCCTCAAACACAAATA |
| Internal bar code: | TCGTCCGTTACGTGCGAACTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 775 |
| LEAP-Seq percent confirming: | 37.5 |
| LEAP-Seq n confirming: | 6 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTGCATGTTCGGCTGGAGA |
| Suggested primer 2: | AGAGAATCCACTTGGCCACG |