| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.002379 |
| Chromosome: | chromosome 17 |
| Location: | 3734065 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g726350 | (1 of 1) IPR000104//IPR002794 - Antifreeze protein, type I // Protein of unknown function DUF92, TMEM19 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCCCCTTTGGCCGTGTTCTTCCGGCCCGTTCTCTCTGTTGATTTTTGCA |
| Internal bar code: | GTAACATTCCCTTAGATTCGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2249 |
| LEAP-Seq percent confirming: | 73.6842 |
| LEAP-Seq n confirming: | 28 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 38 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGAGAATCCACTTGGCCACG |
| Suggested primer 2: | ATTGCATGTTCGGCTGGAGA |