Insertion junction: CLIP2.002662_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.002662
Chromosome:chromosome 1
Location:359450
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g002150 (1 of 5) IPR023220 - Type IV secretion system, VirB5-domain intron
lncRNA_TCONS_00207595 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCTTCGCTGCTCACCCGGCCCTCCTGCGCTCGCAGTCGCCCAAAGGCCG
Internal bar code:ATCCATTTTTACTAAAACATGG

Confirmation - LEAP-Seq

LEAP-Seq distance:1147
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:28
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:28

Suggested primers for confirmation by PCR

Suggested primer 1:ATGTCTTGGGAGCTCGGTTG
Suggested primer 2:CTGCCAGATCTCCTGCTAGC