| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.002673 |
| Chromosome: | chromosome 3 |
| Location: | 4426044 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g175250 | SQS,SQS1 | Squalene synthase; (1 of 1) 2.5.1.21 - Squalene synthase / Squalene synthetase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCTTTGGACCCTCGTCAATCCTACTCATGCTGAAGTGGGGCTGCCTGT |
| Internal bar code: | GATGTCGACATGTTGAGTATAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1065 |
| LEAP-Seq percent confirming: | 95.8333 |
| LEAP-Seq n confirming: | 23 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCAAGGCAGGTGGTATGCT |
| Suggested primer 2: | ATGCAGCTGGTGGGTAAGAC |