| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.002772 |
| Chromosome: | chromosome 3 |
| Location: | 4761205 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g177700 | NIT2 | Transcription factor regulating nitrogen metabolism; (1 of 1) IPR001932//IPR003035 - PPM-type phosphatase domain // RWP-RK domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGACGGCTTCATGACGTCCGTGGAGAAGCGGTAGCGCACCGACACGCAGC |
| Internal bar code: | GAGGGGGTCCATTTATAGACCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2605 |
| LEAP-Seq percent confirming: | 93.0233 |
| LEAP-Seq n confirming: | 40 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 43 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTGTGAACAACCACTGCCG |
| Suggested primer 2: | GCACATGTTCATGACTGCCC |