| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.002808 |
| Chromosome: | chromosome 12 |
| Location: | 7726156 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g554800 | PRK1 | (1 of 1) 2.7.1.19 - Phosphoribulokinase / Phosphopentokinase; Phosphoribulokinase, chloroplastic | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGGCTGCTTGCACGCGCTGCAACTCACCGGAGTCAGCGGCCAGACCGAT |
| Internal bar code: | TTCTCATTATTTATGTTTGGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4826 |
| LEAP-Seq percent confirming: | 97.3333 |
| LEAP-Seq n confirming: | 73 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 75 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCATGGCTTTCACTATGCGC |
| Suggested primer 2: | CACGTGGTTGTAGATGGGCT |