Insertion junction: CLIP2.002815_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.002815
Chromosome:chromosome 16
Location:2758211
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g662150 CPLD51,CCB1 conserved protein required for cyt b6 assembly; (1 of 1) PF12046 - Protein of unknown function (DUF3529) (DUF3529) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCGAAACAAGCCCGCTCGCACGGGCTCCTGCCTCGCGGCTGCCTCGCGG
Internal bar code:TTAAACCCCGTGTGGTGGCTGC

Confirmation - LEAP-Seq

LEAP-Seq distance:494
LEAP-Seq percent confirming:90.9091
LEAP-Seq n confirming:20
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:22

Suggested primers for confirmation by PCR

Suggested primer 1:GACAAGGAGGAGATCGAGCG
Suggested primer 2:CCGCTGAGTTTGAAGTGCAC