Insertion junction: CLIP2.002885_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.002885
Chromosome:chromosome 3
Location:2155482
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g156900 LHCBM5 (1 of 6) K08912 - light-harvesting complex II chlorophyll a/b binding protein 1 (LHCB1); Light-harvesting Chlorophyll a/b binding protein of LHCII 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTTGCGACGGCAGTGCGCGAGCGCGCGGACTGTGGATGGGTTGTATGCTG
Internal bar code:GGGCATAATGTTAACTGTCTTA

Confirmation - LEAP-Seq

LEAP-Seq distance:4303
LEAP-Seq percent confirming:94.3662
LEAP-Seq n confirming:134
LEAP-Seq n nonconfirming:8
LEAP-Seq n unique pos:142

Suggested primers for confirmation by PCR

Suggested primer 1:CGTACATACCGTGGACCGAG
Suggested primer 2:GTCCAAGAAGAGTGGGAGCC