| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.002956 |
| Chromosome: | chromosome 17 |
| Location: | 421825 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g698750 | RAA12,OPR104 | psaA mRNA trans-splicing factor of subcomplex I; (1 of 781) IPR000104 - Antifreeze protein, type I | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCGCCCCCGCCCCCGCCCCCGCCGCATCCTGACTTGCGCCGCGTTGCCC |
| Internal bar code: | AGACATCATAGTCTAGCTCCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 490 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAACGAAGAAGCGCCAGGTA |
| Suggested primer 2: | ATTGTGAACGGTGTGCGTTG |