| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.002996 |
| Chromosome: | chromosome 1 |
| Location: | 1785928 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g009900 | CDJ3 | Chloroplast DnaJ-like protein 3; (1 of 2) IPR001080//IPR001623//IPR017896 - 3Fe-4S ferredoxin // DnaJ domain // 4Fe-4S ferredoxin-type, iron-sulphur binding domain | CDS |
| Cre01.g009950 | NEP1 | NEP1-like protein; (1 of 1) 2.1.1.257 - tRNA (pseudouridine(54)-N(1))-methyltransferase / M(1)-Psi methyltransferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCAATGATGGGTTGGCGCAGCGCCCTCTTGGCTTCCCGACTTGCCCATC |
| Internal bar code: | GCTGGCGAATTGTCTATTAGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4208 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 60 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 60 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCGCGTTTGGTGTACAAA |
| Suggested primer 2: | CCGACATGCCAGATACCTCC |