| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.003004 |
| Chromosome: | chromosome 1 |
| Location: | 6062856 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g042800 | DVR1 | 3%252C8-Divinyl protochlorophyllide a 8-vinyl reductase, chloroplast precursor; (1 of 1) K19073 - divinyl chlorophyllide a 8-vinyl-reductase (DVR) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAGCTGGGCGACCAGGCCGTGTTCGGACAGCAGTAAGGAGTATGGCGAC |
| Internal bar code: | TCTGATCCAGAGTTCGACTTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2427 |
| LEAP-Seq percent confirming: | 89.4737 |
| LEAP-Seq n confirming: | 34 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 38 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACGAAGCAACGAAAACGGT |
| Suggested primer 2: | CTCCTGCTCCACCTTCCTTG |