| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.003085 |
| Chromosome: | chromosome 12 |
| Location: | 565850 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g493950 | PRPS13 | Chloroplast ribosomal protein S13; (1 of 1) K02952 - small subunit ribosomal protein S13 (RP-S13, rpsM) | outside_mRNA |
| Cre12.g494000 | CGL82 | (1 of 2) IPR002083//IPR008974 - MATH/TRAF domain // TRAF-like; Conserved, expressed protein with meprin and TRAF homology domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCGGCCTCAGCTCATGCCGAGCGCCAAGTTGTGCCGCATGTACACAAAC |
| Internal bar code: | GATACAATAAATCTTCAGTGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3764 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 51 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 51 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACTTCAGCAGCGTGGCTAT |
| Suggested primer 2: | CTGGAGGACTTCGCACACAT |