Insertion junction: CLIP2.003172_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.003172
Chromosome:chromosome 12
Location:6034273
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g534700 FKB19 (1 of 1) PTHR10516:SF179 - PEPTIDYL-PROLYL CIS-TRANS ISOMERASE FKBP19, CHLOROPLASTIC; peptidyl-prolyl cis-trans isomerase, FKBP-type 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTGGTTGAGGATCGTGATGGCGACGCAGTGCTGCAGATGAAGCTGCTGC
Internal bar code:CACCAGTCGAACTTTGCTGTAG

Confirmation - LEAP-Seq

LEAP-Seq distance:727
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:CTGGCTAAACTGCAGGAGCT
Suggested primer 2:TTCTTGCGTGCTTTTGCCTC