Insertion junction: CLIP2.003177_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.003177
Chromosome:chromosome 9
Location:3050073
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g393765 LMR1 (1 of 14) PF01476 - LysM domain (LysM); Predicted protein with LysM domain 5'UTR
Cre09.g393802 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTCAAGACGACGACTAGGCTTCCCACTCCGCTTGTTAAGAATGCGCTGTG
Internal bar code:CGTCTTCTTCGGTGATATAGCA

Confirmation - LEAP-Seq

LEAP-Seq distance:1046
LEAP-Seq percent confirming:71.4286
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:TCGATCTCTGCTCCAATCGC
Suggested primer 2:AGCCTTGTCTTCGGAGGTTG