Insertion junction: CLIP2.003222_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.003222
Chromosome:chromosome 4
Location:3793806
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre04.g229550 SRR15 Scavenger receptor cysteine rich (SRCR) protein; (1 of 5) 1.4.3.13 - Protein-lysine 6-oxidase / Lysyl oxidase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACGGCCTGCTGAATGCTGCCACCAGTACTACTGCCTCCGATACTAGAACC
Internal bar code:TCTCAATAGTAAGGACTATATG

Confirmation - LEAP-Seq

LEAP-Seq distance:1036
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:7
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:AAATGGATGCGATGGGTGGT
Suggested primer 2:GTGGCACTGTTGTTCACGAC