| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.003490 |
| Chromosome: | chromosome 7 |
| Location: | 1656533 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g325350 | KIN4-3,KIN10A,KIN10-1 | Kinesin motor protein; (1 of 4) K10395 - kinesin family member 4/21/27 (KIF4_21_27) | 3'UTR |
| Cre07.g325400 | LEU3 | Isopropylmalate dehydrogenase; (1 of 1) 1.1.1.85 - 3-isopropylmalate dehydrogenase / IMDH | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTGTAGGCGAGGCACATCAGGCACCACCACTTGCAGCCATGGTGCCACC |
| Internal bar code: | TTTACCCGGCGAGCAAGGCGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1166 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 10 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGTCTGTGAGCTGGAGGTG |
| Suggested primer 2: | GTATAACGCCGGAGACCCTG |