Gene: Cre07.g325400
Overview
| Common name: | LEU3 |
| Defline: | Isopropylmalate dehydrogenase; (1 of 1) 1.1.1.85 - 3-isopropylmalate dehydrogenase / IMDH |
| Description: | Involved in branched chain amino acid biosynthesis; third committed step in Leu biosynthesis; probably chloroplastic; potential thioredoxin target [PMID: 15123830] |
| Synonyms: | g7516.t1,Cre07.g325400.t1.1,LEU3 |
| Chromosome: | chromosome 7 |
Protein Localization
A plasmid for expressing Cre07.g325400 tagged with the fluorescent protein Venus and affinity epitope 3xFLAG is available: pLM005-Cre07.g325400-Venus-3xFLAG
Imaged strain: CSI_LW08H2
Imaging conditions: Tris-Phosphate media, air, 150 uM photons/m2/s, shaking in 96-well plate; imaged after 16 hours.
Classification(s):
chloroplast/homogeneous/pyrenoid depleted
shapes in cytoplasm/a few small puncta|vesicles in cytoplasm
If you reference these protein localization data in a manuscript, please cite: Wang et al. 2023.
 |  |  |  |
| Venus | Chlorophyll | Bright field | Merged |
Protein-Protein Interactions
We did not determine any protein-protein interactions for this gene.
Mutant Strains
| Insertion Junction | Mutant Strain | Insertion Junction Feature | Confidence (%) | Genome Version |
|---|---|---|---|---|
| CLIP2.003490_1 | CLIP2.003490 | 3'UTR | 80 | 6.1 |
| CLIP2.003490_2 | CLIP2.003490 | 3'UTR | 80 | 6.1 |
| CLIP2.003581_1 | CLIP2.003581 | 3'UTR | 80 | 6.1 |
| CLIP2.003581_2 | CLIP2.003581 | 3'UTR | 80 | 6.1 |
| CLIP2.003828_1 | CLIP2.003828 | 3'UTR | 80 | 6.1 |
| CLIP2.003828_2 | CLIP2.003828 | 3'UTR | 80 | 6.1 |
| LMJ.RY0402.058008_4 | LMJ.RY0402.058008 | intron | 58 | 5.5 |
| LMJ.RY0402.131870_1 | LMJ.RY0402.131870 | 3'UTR | 95 | 5.5 |
| LMJ.RY0402.131870_2 | LMJ.RY0402.131870 | 3'UTR | 95 | 5.5 |
Gene Annotations
| PFAM: | PF00180 |
| PANTHER: | PTHR11835 PTHR11835:SF35 |
| KEGG_ec: | EC:1.1.1.85 |
| KEGG_orthology: | K00052 |
| KOG: | KOG0786 |
| Gene ontology terms:: | GO:0000287, GO:0003862, GO:0005737, GO:0009098, GO:0016616, GO:0051287, GO:0055114 |
| Best arabidopsis TAIR name: | AT1G80560 |
| Best arabidopsis TAIR symbol: | ATIMD2,IMD2 |
| Best arabidopsis TAIR defline: | isopropylmalate dehydrogenase 2 |
| Phytozome Locus Page |
Phenotypes
Our phenotype screens did not find statistically significant results for this gene.