| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.131870 |
| Chromosome: | chromosome 7 |
| Location: | 1665803 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g325400 | LEU3 | Isopropylmalate dehydrogenase; (1 of 1) 1.1.1.85 - 3-isopropylmalate dehydrogenase / IMDH | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCATCGATAACGGTGTAGGCGAGGCACAT |
| Internal bar code: | GGCATTGACATAATCGTGCATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1032 |
| LEAP-Seq percent confirming: | 99.4306 |
| LEAP-Seq n confirming: | 17462 |
| LEAP-Seq n nonconfirming: | 100 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGATGGGATTAGGGGTTTA |
| Suggested primer 2: | GTAGGGTAGGTGCGATGGAA |