| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.003501 |
| Chromosome: | chromosome 3 |
| Location: | 4229065 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g173800 | PDXK1,SOS4,PDX2 | (1 of 1) 2.7.1.35 - Pyridoxal kinase / Vitamin B6 kinase; Pyridoxal kinase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGAAACTGGCTGGCCTCCACTACCACCCCAATAATCTTTATTTGCCTG |
| Internal bar code: | AAAATGGGTGGTGGTCCGGCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3994 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 17 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCACGTCTCTCACCAACT |
| Suggested primer 2: | AACATATTGCAATCGGGCGC |