Insertion junction: CLIP2.003543_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.003543
Chromosome:chromosome 6
Location:5369827
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g285250 LHCBM6 (1 of 6) K08912 - light-harvesting complex II chlorophyll a/b binding protein 1 (LHCB1); Light-harvesting Chloropyll a/b binding protein of LHCII type I, chloroplast precursor intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTGTCCGCCCTGGACGCATCTATTTGCCCTTCCGACCAGCGCCACAACT
Internal bar code:CTACCCATCTGAGGTAATCCTA

Confirmation - LEAP-Seq

LEAP-Seq distance:1873
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:AACGCATTTGCAGTTGGGTC
Suggested primer 2:ACGCCGCTTGCATGATTTAC