Insertion junction: CLIP2.003623_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.003623
Chromosome:chromosome 13
Location:2934824
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g583550 VIP1,VIPP1 (1 of 2) K03969 - phage shock protein A (pspA); Vesicle inducing protein in plastids 1 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATCTGCGAGCATTCACGCGCGAGGGTTGCAGGTTAGGTTCTTGGGGGGAG
Internal bar code:GCCTGGCAGGATCGCAGCGTGT

Confirmation - LEAP-Seq

LEAP-Seq distance:2862
LEAP-Seq percent confirming:93.1034
LEAP-Seq n confirming:27
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:29

Suggested primers for confirmation by PCR

Suggested primer 1:ACGCAAAAATGTCGCTGCTT
Suggested primer 2:CTCCCAACCTGACTGACACC