| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.003687 |
| Chromosome: | chromosome 9 |
| Location: | 933331 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g402950 | CGL21 | Conserved in the Green Lineage | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTGTTCAGACGTCCTTGGTCATCTACTTAAGCCTCCGCCCTAGTCCTCA |
| Internal bar code: | TGCTTGAATACGTTGCGGGATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 882 |
| LEAP-Seq percent confirming: | 32.5 |
| LEAP-Seq n confirming: | 13 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 40 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTACGAGGCGGAACTTGAC |
| Suggested primer 2: | TCGACTGGTTGTTTCCTCCG |