| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.003708 |
| Chromosome: | chromosome 2 |
| Location: | 7092583 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g390060 | RAA4 | psaA mRNA trans-splicing factor | outside_mRNA |
| Cre09.g390097 | (1 of 112) 2.7.11.25 - Mitogen-activated protein kinase kinase kinase / MLTK | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGAATGTTTCGGTACCGTGAATGTGTTCCTCTTGTATCCCGCGCTTGGT |
| Internal bar code: | TGGGATGTAACAGTGACAATGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4934 |
| LEAP-Seq percent confirming: | 96.7033 |
| LEAP-Seq n confirming: | 88 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 91 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGAACATTTGTTGGGCAGCG |
| Suggested primer 2: | GCAGGGCAACTATGAATGCG |