| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.003708 |
| Chromosome: | chromosome 2 |
| Location: | 7092584 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g390060 | RAA4 | psaA mRNA trans-splicing factor | outside_mRNA |
| Cre09.g390097 | (1 of 112) 2.7.11.25 - Mitogen-activated protein kinase kinase kinase / MLTK | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGCGTCAAGCGCCCTGAGCGCAGCGATTCAGTGGGGACGACGCCTCTCA |
| Internal bar code: | ATCGGCTTCACATCGGCTACTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 6663 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 75 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 75 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGGGCAACTATGAATGCG |
| Suggested primer 2: | TGAACATTTGTTGGGCAGCG |