Insertion junction: CLIP2.003835_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.003835
Chromosome:chromosome 9
Location:2274898
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g394750 PRPS1 (1 of 2) PTHR10724 - S1 RNA-BINDING DOMAIN-CONTAINING PROTEIN 1; Chloroplast Ribosomal Protein S1 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTACAAGCAGGCGCACCCAGCTGCCGCGGCGCGTCGGCTGCTGCAAGCAC
Internal bar code:CGGGGTAGCATCGGATATCATG

Confirmation - LEAP-Seq

LEAP-Seq distance:2998
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:19
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:19

Suggested primers for confirmation by PCR

Suggested primer 1:ACGCTAAGCACCAAGAAGCT
Suggested primer 2:TTTGTCTCTCGGCCTGGTTC