Insertion junction: CLIP2.003847_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.003847
Chromosome:chromosome 1
Location:4085493
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g027150 CPLD46,HEL5 Conserved in the plant lineage and diatoms; (1 of 2) PTHR24031//PTHR24031:SF249 - RNA HELICASE // ATP-DEPENDENT DNA HELICASE RECG CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCGCTGTCCGCCGCGCGCATCACTTCCATCTTCCCCTTCCCACTGGACG
Internal bar code:GCAGAGCAGTTTAAAGGTCACT

Confirmation - LEAP-Seq

LEAP-Seq distance:983
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:8
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:ACTACCCCTACCCCATTCCC
Suggested primer 2:TGTAACTGAGTCACCGTGGC