| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.003855 |
| Chromosome: | chromosome 14 |
| Location: | 1923759 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g620300 | ASB1,ANS2 | Anthranilate synthase, beta subunit; (1 of 1) K01658 - anthranilate synthase component II (trpG) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGGTGCACAACTTGCATGATGGGGGCCCGGTCGGCATTTAGTCAAAGCG |
| Internal bar code: | TACTCTAAGCCAAGAAACGACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3908 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 73 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 73 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTCCTCACCTAGCCCTCTT |
| Suggested primer 2: | TCCACGGTTTTCTCGTCGTT |