| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.003886 |
| Chromosome: | chromosome 3 |
| Location: | 6541683 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g194200 | PDH2 | Pyruvate dehydrogenase E1 beta subunit; (1 of 1) PTHR11624//PTHR11624:SF76 - DEHYDROGENASE RELATED // PYRUVATE DEHYDROGENASE E1 COMPONENT SUBUNIT BETA-2, CHLOROPLASTIC-RELATED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTAACCCCATCATCCCGCTCATCACCTCCTGCCCGTTTCCGTGTTTCTCG |
| Internal bar code: | GGCCGTGTATTGGGTAGAATGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1271 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 20 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACCATCCACGTACACGCTT |
| Suggested primer 2: | ACAGTTCAAGGTGGGCACAA |