| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.003930 |
| Chromosome: | chromosome 10 |
| Location: | 2258949 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g433950 | (1 of 2) PTHR33222:SF3 - PROTEIN CURVATURE THYLAKOID 1C, CHLOROPLASTIC | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTAAACCATGCTAATACCGTCTTTCTCGGACTTTTGCAAATGCTCACCG |
| Internal bar code: | GTAATTTCTTTGTTTTGCATCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2511 |
| LEAP-Seq percent confirming: | 85.1351 |
| LEAP-Seq n confirming: | 63 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 74 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGATCCCCATCGTGAGTCCA |
| Suggested primer 2: | CAGAATTGCACACGGCCAAA |