Insertion junction: CLIP2.003965_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.003965
Chromosome:chromosome 3
Location:6023507
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g189800 CYN38 Cyclophilin 38; (1 of 1) PTHR11071//PTHR11071:SF236 - PEPTIDYL-PROLYL CIS-TRANS ISOMERASE // PEPTIDYL-PROLYL CIS-TRANS ISOMERASE CYP38, CHLOROPLASTIC outside_mRNA

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AAGTAGGTGGCTGTATACGGACCTCGTTTGTGCCTGCCACTCACGATTTT
Internal bar code:TACCCTTATGTCGATCTGAGCT

Confirmation - LEAP-Seq

LEAP-Seq distance:3003
LEAP-Seq percent confirming:98.8636
LEAP-Seq n confirming:87
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:88

Suggested primers for confirmation by PCR

Suggested primer 1:GTCTTGTGCAGTTGCCCTTG
Suggested primer 2:ACATACCTACCCAGGAGCGT