Insertion junction: CLIP2.004037_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.004037
Chromosome:chromosome 8
Location:1649641
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre08.g365900 LHCSR1,LI818 (1 of 3) PTHR21649//PTHR21649:SF15 - CHLOROPHYLL A/B BINDING PROTEIN // SUBFAMILY NOT NAMED; Stress-related chlorophyll a/b binding protein 1 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CATACCATGCCTGCCACTAAACAAACTCGTGCCTGACTGTCGCGCCATCG
Internal bar code:GGTATGGCATCGTCGTAGGACT

Confirmation - LEAP-Seq

LEAP-Seq distance:1981
LEAP-Seq percent confirming:98.4615
LEAP-Seq n confirming:64
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:65

Suggested primers for confirmation by PCR

Suggested primer 1:CCAACAGCGCGTGTGTAAAA
Suggested primer 2:CTCCCCACCCAACAGACTTC