| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.004205 |
| Chromosome: | chromosome 2 |
| Location: | 3377962 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g098300 | (1 of 1) K11547 - kinetochore protein NDC80 (NDC80, HEC1, TID3) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTGTGTGTGTGTGTGTGATTAACGCGTGTTGTGTTGCTGTTGTGGGGCC |
| Internal bar code: | GGAGTATGGCTAATATTGGGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1820 |
| LEAP-Seq percent confirming: | 96.6667 |
| LEAP-Seq n confirming: | 58 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 60 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCACGTTCCTCTTCCAGCA |
| Suggested primer 2: | CCTCCCACCCTGATACCAGA |