Insertion junction: CLIP2.004248_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.004248
Chromosome:chromosome 3
Location:2154499
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g156900 LHCBM5 (1 of 6) K08912 - light-harvesting complex II chlorophyll a/b binding protein 1 (LHCB1); Light-harvesting Chlorophyll a/b binding protein of LHCII CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCCTTCACCTTCAGCTCAGCGAAAGCATCGGGGTCCTCAGCCAGGCCCA
Internal bar code:GGTCGGCGCTGTCGCTACGGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:3169
LEAP-Seq percent confirming:95.0
LEAP-Seq n confirming:38
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:40

Suggested primers for confirmation by PCR

Suggested primer 1:AAGAACGGTGCGTGATTTGC
Suggested primer 2:CTCGGTCCACGGTATGTACG