| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.004270 |
| Chromosome: | chromosome 2 |
| Location: | 3377146 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g098300 | (1 of 1) K11547 - kinetochore protein NDC80 (NDC80, HEC1, TID3) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGACGGCGTACCGGCTCACACCATGCGCGTCAGCACTCTCATGGGACTC |
| Internal bar code: | CCTTTATGACTACAATTTACGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3239 |
| LEAP-Seq percent confirming: | 87.8049 |
| LEAP-Seq n confirming: | 36 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 41 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCAACACAACACGCGTTA |
| Suggested primer 2: | TATACACGCGCTACAGGCTG |