Insertion junction: CLIP2.004290_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.004290
Chromosome:chromosome 3
Location:6024345
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g189800 CYN38 Cyclophilin 38; (1 of 1) PTHR11071//PTHR11071:SF236 - PEPTIDYL-PROLYL CIS-TRANS ISOMERASE // PEPTIDYL-PROLYL CIS-TRANS ISOMERASE CYP38, CHLOROPLASTIC CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAGCGTGAAGTCGTAAATGGGCTCCTTGTCGCCCTGCACGCGCACCTCGA
Internal bar code:AGCTACGAAAGACGTGTGCTCG

Confirmation - LEAP-Seq

LEAP-Seq distance:319
LEAP-Seq percent confirming:66.6667
LEAP-Seq n confirming:6
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:GATGTGGATGTGGGAGGGTG
Suggested primer 2:CGAGGTCCGTATACAGCCAC