Insertion junction: CLIP2.004310_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.004310
Chromosome:chromosome 2
Location:6233021
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g387000 CGL34 (1 of 4) PF11891 - Domain of unknown function (DUF3411) (DUF3411); Conserved in the Green Lineage CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAATGGCCCCACTCGACTGCTGTGCTACAAGCGACGCCAGCTCCGATCCC
Internal bar code:CTGTATCGGTATTCGGTGATTG

Confirmation - LEAP-Seq

LEAP-Seq distance:4194
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:122
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:122

Suggested primers for confirmation by PCR

Suggested primer 1:CAGCAACTTCCTCTGGACGT
Suggested primer 2:AAGGCGAGAGTAGGGTCAGT