| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.004313 |
| Chromosome: | chromosome 10 |
| Location: | 5320448 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g456650 | AOF6 | (1 of 1) 1.4.3.4 - Monoamine oxidase / Tyramine oxidase; Flavin-containing amine oxidase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGGCCAAGGGTTGATCCGCTGCCCCTGAATCCTGGAGCCATTGCTGCCG |
| Internal bar code: | GCTAAATGGGACAATTAAGTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2814 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 70 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 70 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCATTCGGATTCTCGTGTGC |
| Suggested primer 2: | TGATGTGGTTGTGATGGCGA |