| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.004424 |
| Chromosome: | chromosome 7 |
| Location: | 2470375 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g329300 | MSC1 | Mechanosensitive ion channel; (1 of 1) PTHR30221 - SMALL-CONDUCTANCE MECHANOSENSITIVE CHANNEL | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCACCTCCTCACTTCCCCCTTTATCTCCATGCTGGGTGGCGAGAGCAGC |
| Internal bar code: | ACCAGGACATGTGTACTCAATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1758 |
| LEAP-Seq percent confirming: | 85.2941 |
| LEAP-Seq n confirming: | 29 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 34 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCCACGCATACGAGATACA |
| Suggested primer 2: | TCTCGGTATCGCCAATCACG |