| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.004483 |
| Chromosome: | chromosome 1 |
| Location: | 7011898 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g050100 | DPD1 | Diaminopimelate decarboxylase; (1 of 1) K01586 - diaminopimelate decarboxylase (lysA) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGATGTCGAACCTTGTATGTCCATGTCATGCCTGTAGGCAAGGGTCTGGG |
| Internal bar code: | TAATGTATGAATGTAGTTGAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 504 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGGGAAAGCTCACGGAAGG |
| Suggested primer 2: | CGATTGCTGCCCGTGTTATG |