Insertion junction: CLIP2.004533_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.004533
Chromosome:chromosome 12
Location:8615000
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g548400 LHCBM2 (1 of 3) K08913 - light-harvesting complex II chlorophyll a/b binding protein 2 (LHCB2); Light-harvesting protein of photosystem II CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAGCGCGGGGCACCACGCGGGCGGACTGTCAAAAGCGGAGCAGGAAGGCA
Internal bar code:CAACGCCGATGATCGGGCAGCC

Confirmation - LEAP-Seq

LEAP-Seq distance:2833
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:67
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:67

Suggested primers for confirmation by PCR

Suggested primer 1:ACTCGTTCTACGCGCCTATG
Suggested primer 2:TGGATCAGGTTCTCGTTGCC