Insertion junction: CLIP2.004550_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.004550
Chromosome:chromosome 9
Location:3047737
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g393728 (1 of 3) K05302 - SET domain-containing protein 6 (SETD6) 3'UTR
Cre09.g393765 LMR1 (1 of 14) PF01476 - LysM domain (LysM); Predicted protein with LysM domain 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACTCCGTGCGAGTCTTATGCGGCAGTCCAAGGGCAGCGCTGTCCGCGCTA
Internal bar code:GCCTCATTTGAGCAGTGCAATC

Confirmation - LEAP-Seq

LEAP-Seq distance:1503
LEAP-Seq percent confirming:62.5
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:ACCCTGACTCGCCCTTATCT
Suggested primer 2:TGCGGATTGCCAAACAACTG