| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.004598 |
| Chromosome: | chromosome 3 |
| Location: | 6545691 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g194200 | PDH2 | Pyruvate dehydrogenase E1 beta subunit; (1 of 1) PTHR11624//PTHR11624:SF76 - DEHYDROGENASE RELATED // PYRUVATE DEHYDROGENASE E1 COMPONENT SUBUNIT BETA-2, CHLOROPLASTIC-RELATED | 3'UTR |
| Cre03.g194250 | (1 of 1) K05609 - ubiquitin carboxyl-terminal hydrolase L3 [EC:3.4.19.12] (UCHL3, YUH1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACACATGATTAGACTCTCGCCCCGCCACCCGGCTCCTCCCAGCTTCGTG |
| Internal bar code: | TGGTTAAAACGCCCCATAAGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1066 |
| LEAP-Seq percent confirming: | 96.7742 |
| LEAP-Seq n confirming: | 30 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCCTTGCCGATGAGGATAA |
| Suggested primer 2: | GATAGGAGGCAGTAGCAGCG |